![]() The backcrossed genotypes with Striga resistance allowed fewer Striga plants to germinate though in certain cases Ochuti genotypes performed equally the same. ![]() Eight progenies from BC2F1 and BC3F1 backcross populations were evaluated in field trials under artificial Striga inoculation in two locations and for two seasons. Eleven polymorphic markers identified at least three Striga resistance QTLs, in five plants of BC3F1//F2 generations. At the BC3F1 stage, foreground selection for the Striga resistance Quantitative Trait Loci (QTLs) was conducted through Polymerase Chain Reaction (PCR) and N13 and Ochuti alleles sized through capillary electrophoresis. Two backcross populations namely, BC2F1 and BC3F1 were generated by crossing N13, the donor parent to Ochuti, the recurrent parent line and the resultant backcrossed Striga resistant progenies were subjected to phenotypic selection initially. The aim of this study was to introgress Striga resistance from a documented resistant donor line N13, into Ochuti, a susceptible farmer preferred variety through molecular marker assisted selection (MAS). In the marginal and semi-arid areas, there is urgent need to enhance the genetic resistance to Striga hermonthica in local varieties. A B S T R A C T Sorghum, the second most important cereal crop in Kenya is often attacked by Striga hermonthica weed with grain yields being reduced up to 100%.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |